|
KMID : 0895420030130030234
|
|
Journal of Korean Society of Occupational and Enviromental Hygiene
2003 Volume.13 No. 3 p.234 ~ p.253
|
|
|
Comparison of Methods for Identification and the Effects on Legionella pneumophila of the Cooling Towers in Seoul
|
|
Kim Key-Young
Kim Yoon-Shin
Song Jae-Chul
Lee Soo-Jin
Kim Sung-Uk
Choi Tae-Yeal
Park Won-Seok
Lee Cheol-Min
|
|
|
Abstract
|
|
|
In order to prevent and control Legionellosis outbreaks, we determined level of contamination with Legionellae and the effects on Legionella spp. of the cooling towers in Seoul City. This study was carried out to compare the Legionella spp. for 68 samples of cooling tower by conventional culture method with there samples by Multiplex PCR(polymerase chain reaction) from July to August in 2002. Also it was analyzed the distribution of Legionella subtypes, and evaluated molecular typing methods discrimination function and feasibility, using a arbitrarily primed(Ap)-PCR with M13 reverse primer.
The detection positive rate of Legionella spp. was 22(32.3%) by conventional culture method and 33(48.5%) by multiplex PCR method of 68 samples. Multiplex PCR amplification showed 100% of positivity for 22 samples. Out of 22 Legionella isolates, 19 were identified as Legionella pneumophilla serogroup I and 3 as Legionella pneumophilla Ⅳ. Molecular analysis of 30 Legionella pneu- mophilla serogroup I showed 5 subtypes (Ia in isolates 17, Ib, 2; Ic, 7; Ⅱ,2 ; Ⅲ, 2) and Legionella pneumophilla Ⅳ showed( VI in isolates 2 Ⅴ, 1) by Ap-PCR. Most of the effectives except turbidity seemed not to be significant with Legionella species isolation and it was significant to do not disinfect in multiplex PCR result.
This results suggest that PCR method could be contributed to detection of Legionella spp. Also it showed that the Ap-PCR method could be used as a rapid and reproducible tool in tracking legionellosis outbreak.
|
|
|
KEYWORD
|
|
|
Legionella pneumophilla, Cooling tower, Legionellosis, Conventional culture, Serogroup, multiplex PCR, The effectives, Ap-PCR
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|